Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739016..739791 Replicon chromosome
Accession NZ_CP061399
Organism Klebsiella pneumoniae strain NU-CRE085

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HCV82_RS03690 Protein ID WP_004150910.1
Coordinates 739306..739791 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HCV82_RS03685 Protein ID WP_004150912.1
Coordinates 739016..739309 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV82_RS03665 734224..734826 - 603 WP_002916735.1 short chain dehydrogenase -
HCV82_RS03670 734924..735835 + 912 WP_004188550.1 LysR family transcriptional regulator -
HCV82_RS03675 735836..736984 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HCV82_RS03680 736995..738371 - 1377 WP_004217775.1 cystathionine beta-synthase -
HCV82_RS03685 739016..739309 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HCV82_RS03690 739306..739791 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HCV82_RS03695 740495..741088 + 594 WP_004188553.1 hypothetical protein -
HCV82_RS03700 741185..741401 + 217 Protein_727 transposase -
HCV82_RS03705 742007..742879 + 873 WP_004188557.1 ParA family protein -
HCV82_RS03710 742879..743262 + 384 WP_004150906.1 hypothetical protein -
HCV82_RS03715 743255..744622 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741185..741337 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T172611 WP_004150910.1 NZ_CP061399:739306-739791 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T172611 NZ_CP079819:628357-628611 [Rothia aeria]
ATGAAAACCATGTCTTACACTGAGTCTCGGGCTCGGTATGCTGAAGTACTTGATCACGTTGAAGACCACCGCGAAGAAGT
TGTCATCACTCGATCCGGGCATGAGCCTGCAGTTATTCTCTCATTGTCTGAGTACGAATCTCTTCAAGAGACTGCTTACC
TTATGCGTTCCCCGGCTAACGCACGTCGTCTTATGGAGTCTATTTCTCGTCTTGAAGCTGGTCTTGGAACTGAGCAGCAG
CTGATTGAGGAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT172611 WP_004150912.1 NZ_CP061399:739016-739309 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT172611 NZ_CP079819:628614-628874 [Rothia aeria]
GTGAGAATCATATTTGATGATTCAGCATGGAGCGACTATCTCTGGTGGCAGGCTCAGGATCGGAAGGTTCTCAAACGTAT
TAATCTACTCATTAAAGATGTTGAGCGAAACGGTAACGAAGGCATAGGGAAGCCTGAAGCTCTCAGACACCAATTCTCAG
GTTGGTGGTCACGCAGAATTACCGATGAACACCGTCTCGTATACAAGCTTACTGACGACTCTATCGTTATAGCTTCATGC
CGATATCACTATGGAGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References