Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3431470..3432245 Replicon chromosome
Accession NZ_CP107297
Organism Klebsiella pneumoniae strain CRKP_44

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag OH517_RS17300 Protein ID WP_004150910.1
Coordinates 3431470..3431955 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag OH517_RS17305 Protein ID WP_004150912.1
Coordinates 3431952..3432245 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH517_RS17275 3426639..3428006 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
OH517_RS17280 3427999..3428382 - 384 WP_004150906.1 hypothetical protein -
OH517_RS17285 3428382..3429254 - 873 WP_004188557.1 ParA family protein -
OH517_RS17290 3429860..3430076 - 217 Protein_3396 transposase -
OH517_RS17295 3430173..3430766 - 594 WP_004188553.1 hypothetical protein -
OH517_RS17300 3431470..3431955 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
OH517_RS17305 3431952..3432245 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
OH517_RS17310 3432890..3434266 + 1377 WP_004217775.1 cystathionine beta-synthase -
OH517_RS17315 3434277..3435425 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
OH517_RS17320 3435426..3436337 - 912 WP_004188550.1 LysR family transcriptional regulator -
OH517_RS17325 3436435..3437037 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3373250..3432496 59246
flank IS/Tn - - 3429924..3430076 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T260661 WP_004150910.1 NZ_CP107297:c3431955-3431470 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT260661 WP_004150912.1 NZ_CP107297:c3432245-3431952 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References