Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3642097..3642872 Replicon chromosome
Accession NZ_CP074580
Organism Klebsiella pneumoniae strain B30155

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag KH752_RS17510 Protein ID WP_004889762.1
Coordinates 3642387..3642872 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KH752_RS17505 Protein ID WP_004150912.1
Coordinates 3642097..3642390 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH752_RS17485 (KH752_17485) 3637306..3637908 - 603 WP_004174410.1 short chain dehydrogenase -
KH752_RS17490 (KH752_17490) 3638006..3638917 + 912 WP_017879796.1 LysR family transcriptional regulator -
KH752_RS17495 (KH752_17495) 3638918..3640066 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
KH752_RS17500 (KH752_17500) 3640077..3641453 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
KH752_RS17505 (KH752_17505) 3642097..3642390 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KH752_RS17510 (KH752_17510) 3642387..3642872 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
KH752_RS17515 (KH752_17515) 3643576..3644168 + 593 Protein_3427 hypothetical protein -
KH752_RS17520 (KH752_17520) 3644265..3644481 + 217 Protein_3428 transposase -
KH752_RS17530 (KH752_17530) 3644996..3645709 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3644265..3644417 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T202467 WP_004889762.1 NZ_CP074580:3642387-3642872 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T202467 NZ_CP099413:877069-877476 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT202467 WP_004150912.1 NZ_CP074580:3642097-3642390 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT202467 NZ_CP099413:876822-877088 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References