Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741316..742091 Replicon chromosome
Accession NZ_CP066254
Organism Klebsiella pneumoniae strain CRKP78R

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JCR35_RS03700 Protein ID WP_004150910.1
Coordinates 741606..742091 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JCR35_RS03695 Protein ID WP_004150912.1
Coordinates 741316..741609 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR35_RS03675 736524..737126 - 603 WP_062954968.1 short chain dehydrogenase -
JCR35_RS03680 737224..738135 + 912 WP_004188550.1 LysR family transcriptional regulator -
JCR35_RS03685 738136..739284 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JCR35_RS03690 739295..740671 - 1377 WP_004217775.1 cystathionine beta-synthase -
JCR35_RS03695 741316..741609 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JCR35_RS03700 741606..742091 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JCR35_RS03705 742795..743388 + 594 WP_004188553.1 hypothetical protein -
JCR35_RS03710 743485..743701 + 217 Protein_729 transposase -
JCR35_RS03715 744307..745179 + 873 WP_004188557.1 ParA family protein -
JCR35_RS03720 745179..745562 + 384 WP_004150906.1 hypothetical protein -
JCR35_RS03725 745555..746922 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743485..743637 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T184228 WP_004150910.1 NZ_CP066254:741606-742091 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T184228 NZ_CP087576:c18056-17778 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT184228 WP_004150912.1 NZ_CP066254:741316-741609 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT184228 NZ_CP087576:c18325-18056 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References