214231

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1023721..1024496 Replicon chromosome
Accession NZ_CP082154
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6977_RS04885 Protein ID WP_004150910.1
Coordinates 1023721..1024206 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6977_RS04890 Protein ID WP_004150912.1
Coordinates 1024203..1024496 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS04860 (K6977_04855) 1018890..1020257 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
K6977_RS04865 (K6977_04860) 1020250..1020633 - 384 WP_004150906.1 hypothetical protein -
K6977_RS04870 (K6977_04865) 1020633..1021505 - 873 WP_004188557.1 ParA family protein -
K6977_RS04875 (K6977_04870) 1022111..1022327 - 217 Protein_951 transposase -
K6977_RS04880 (K6977_04875) 1022424..1023017 - 594 WP_004188553.1 hypothetical protein -
K6977_RS04885 (K6977_04880) 1023721..1024206 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6977_RS04890 (K6977_04885) 1024203..1024496 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6977_RS04895 (K6977_04890) 1025141..1026517 + 1377 WP_004217775.1 cystathionine beta-synthase -
K6977_RS04900 (K6977_04895) 1026528..1027676 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6977_RS04905 (K6977_04900) 1027677..1028588 - 912 WP_004188550.1 LysR family transcriptional regulator -
K6977_RS04910 (K6977_04905) 1028686..1029288 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 965393..1024747 59354
flank IS/Tn - - 1022175..1022327 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T214231 WP_004150910.1 NZ_CP082154:c1024206-1023721 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T214231 NZ_CP110016:c2178480-2178226 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT214231 WP_004150912.1 NZ_CP082154:c1024496-1024203 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT214231 NZ_CP110016:c2178728-2178477 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References