Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4486968..4487743 Replicon chromosome
Accession NZ_CP090242
Organism Klebsiella pneumoniae strain KPNSF-2

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LXH14_RS21540 Protein ID WP_004889762.1
Coordinates 4487258..4487743 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LXH14_RS21535 Protein ID WP_004150912.1
Coordinates 4486968..4487261 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH14_RS21515 (4482177) 4482177..4482779 - 603 WP_002916735.1 short chain dehydrogenase -
LXH14_RS21520 (4482877) 4482877..4483788 + 912 WP_014906929.1 LysR family transcriptional regulator -
LXH14_RS21525 (4483789) 4483789..4484937 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
LXH14_RS21530 (4484948) 4484948..4486324 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
LXH14_RS21535 (4486968) 4486968..4487261 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LXH14_RS21540 (4487258) 4487258..4487743 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LXH14_RS21545 (4488447) 4488447..4489040 + 594 WP_004188553.1 hypothetical protein -
LXH14_RS21550 (4489137) 4489137..4489353 + 217 Protein_4215 transposase -
LXH14_RS21560 (4490031) 4490031..4490744 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4489137..4489289 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T230336 WP_004889762.1 NZ_CP090242:4487258-4487743 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T230336 NZ_CP124873:324492-324860 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT230336 WP_004150912.1 NZ_CP090242:4486968-4487261 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT230336 NZ_CP124873:324274-324495 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References