Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3246695..3247470 Replicon chromosome
Accession NZ_CP022882
Organism Klebsiella pneumoniae strain 911021

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CJU70_RS16390 Protein ID WP_004150910.1
Coordinates 3246985..3247470 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CJU70_RS16385 Protein ID WP_004150912.1
Coordinates 3246695..3246988 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU70_RS16365 3241903..3242505 - 603 WP_062954968.1 short chain dehydrogenase -
CJU70_RS16370 3242603..3243514 + 912 WP_004188550.1 LysR family transcriptional regulator -
CJU70_RS16375 3243515..3244663 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CJU70_RS16380 3244674..3246050 - 1377 WP_004217775.1 cystathionine beta-synthase -
CJU70_RS16385 3246695..3246988 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CJU70_RS16390 3246985..3247470 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CJU70_RS16395 3248174..3248767 + 594 WP_004188553.1 hypothetical protein -
CJU70_RS16400 3248864..3249080 + 217 Protein_3092 transposase -
CJU70_RS16415 3249686..3250558 + 873 WP_004188557.1 ParA family protein -
CJU70_RS16420 3250558..3250941 + 384 WP_004150906.1 hypothetical protein -
CJU70_RS16425 3250934..3252301 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3248864..3249016 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T79838 WP_004150910.1 NZ_CP022882:3246985-3247470 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T79838 NZ_CP022882:3246985-3247470 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT79838 WP_004150912.1 NZ_CP022882:3246695-3246988 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT79838 NZ_CP022882:3246695-3246988 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References