Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 735170..735945 Replicon chromosome
Accession NZ_CP079781
Organism Klebsiella pneumoniae subsp. pneumoniae strain T698-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KYY07_RS03675 Protein ID WP_004150910.1
Coordinates 735460..735945 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KYY07_RS03670 Protein ID WP_004150912.1
Coordinates 735170..735463 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYY07_RS03650 (KYY07_03650) 730378..730980 - 603 WP_002916735.1 short chain dehydrogenase -
KYY07_RS03655 (KYY07_03655) 731078..731989 + 912 WP_004188550.1 LysR family transcriptional regulator -
KYY07_RS03660 (KYY07_03660) 731990..733138 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KYY07_RS03665 (KYY07_03665) 733149..734525 - 1377 WP_004217775.1 cystathionine beta-synthase -
KYY07_RS03670 (KYY07_03670) 735170..735463 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KYY07_RS03675 (KYY07_03675) 735460..735945 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KYY07_RS03680 (KYY07_03680) 736649..737242 + 594 WP_004188553.1 hypothetical protein -
KYY07_RS03685 (KYY07_03685) 737339..737555 + 217 Protein_724 transposase -
KYY07_RS03690 (KYY07_03690) 738161..739033 + 873 WP_004188557.1 ParA family protein -
KYY07_RS03695 (KYY07_03695) 739033..739416 + 384 WP_004150906.1 hypothetical protein -
KYY07_RS03700 (KYY07_03700) 739409..740776 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 737339..737491 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T210084 WP_004150910.1 NZ_CP079781:735460-735945 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T210084 NZ_CP104386:2733513-2733626 [Cupriavidus gilardii]
ATCGGTACCCGCCTGCACTGTCTGATCTTTACCATACGAGGCGACACGCTGCGGGCCATCAGTCTACGCAAAGCCAATTT
CCGAGAGGTGCGAGACTATGAACAGGAAATCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT210084 WP_004150912.1 NZ_CP079781:735170-735463 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT210084 NZ_CP104386:2733610-2733963 [Cupriavidus gilardii]
ATGAACAGGAAATCTAAGCTGACCATGCCGGCCGCCGACGAGAACGCCGCCATCGCGCGCGGCATTGCGGACGATCCGGA
TGCGGTCGAACTGACGACCGAGCAGATCAAGCGTATGCGTCCGGCACGTGAAGCATTGGCCGAAGTGCTTGGCGAGAGAA
ACGTTGAGGCGCTGATCAAGCGACGGGGCCGCCCTGCCCTGCCGGCGGCCGAACGCAAAGTCAGTCAAACCCTGCGACTC
GATCCTGACGTGCTGCAGGCATTCAAGGCAACCGGCGACGGGTGGCAAACGCGCATCAATGATGCGCTGCGCGCCTATGC
CAAGTCTCATCGGATGCTGCCGCGCGGCTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References