Detailed information of TA system TA229798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 727920..728695
• Replicon: chromosome
• Accession: NZ_CP089959
• Organism: Klebsiella pneumoniae strain KP403

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LVQ74_RS03610
• Protein ID: WP_060568463.1
• Coordinates: 728210..728695 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: LVQ74_RS03605
• Protein ID: WP_004150912.1
• Coordinates: 727920..728213 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ74_RS03585 (LVQ74_03585)	723128..723730	-	603	WP_004174410.1	short chain dehydrogenase	-
LVQ74_RS03590 (LVQ74_03590)	723828..724739	+	912	WP_023342207.1	LysR family transcriptional regulator	-
LVQ74_RS03595 (LVQ74_03595)	724740..725888	-	1149	WP_016947441.1	PLP-dependent aspartate aminotransferase family protein	-
LVQ74_RS03600 (LVQ74_03600)	725899..727275	-	1377	WP_004174417.1	pyridoxal-phosphate dependent enzyme	-
LVQ74_RS03605 (LVQ74_03605)	727920..728213	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
LVQ74_RS03610 (LVQ74_03610)	728210..728695	+	486	WP_060568463.1	GNAT family N-acetyltransferase	Toxin
LVQ74_RS03615 (LVQ74_03615)	729399..729992	+	594	WP_004188553.1	hypothetical protein	-
LVQ74_RS03620 (LVQ74_03620)	730089..730305	+	217	Protein_710	transposase	-
LVQ74_RS03630 (LVQ74_03630)	731472..732185	-	714	WP_002916694.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_016845 Klebsiella pneumoniae subsp. pneumoniae HS11286 IS/Tn TA026030 4559372..4559524 0.008	flank	IS/Tn	-	-	730089..730241	152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17602.64 Da
• Isoelectric Point: 8.1629

>T229798 WP_060568463.1 NZ_CP089959:728210-728695 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRHNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T229798 NZ_CP124517:c4581498-4581280 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT229798 WP_004150912.1 NZ_CP089959:727920-728213 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT229798 NZ_CP124517:c4581898-4581524 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4