Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3103869..3104644 Replicon chromosome
Accession NZ_CP095253
Organism Klebsiella pneumoniae strain 21080937

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MU320_RS15145 Protein ID WP_004150910.1
Coordinates 3104159..3104644 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MU320_RS15140 Protein ID WP_004150912.1
Coordinates 3103869..3104162 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU320_RS15120 (MU320_15120) 3099077..3099679 - 603 WP_062954968.1 short chain dehydrogenase -
MU320_RS15125 (MU320_15125) 3099777..3100688 + 912 WP_004188550.1 LysR family transcriptional regulator -
MU320_RS15130 (MU320_15130) 3100689..3101837 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MU320_RS15135 (MU320_15135) 3101848..3103224 - 1377 WP_004217775.1 cystathionine beta-synthase -
MU320_RS15140 (MU320_15140) 3103869..3104162 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MU320_RS15145 (MU320_15145) 3104159..3104644 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MU320_RS15150 (MU320_15150) 3105348..3105941 + 594 WP_004188553.1 hypothetical protein -
MU320_RS15155 (MU320_15155) 3106038..3106254 + 217 Protein_2952 transposase -
MU320_RS15160 (MU320_15160) 3106860..3107732 + 873 WP_004188557.1 ParA family protein -
MU320_RS15165 (MU320_15165) 3107732..3108115 + 384 WP_004150906.1 hypothetical protein -
MU320_RS15170 (MU320_15170) 3108108..3109475 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3106038..3106190 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T241788 WP_004150910.1 NZ_CP095253:3104159-3104644 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T241788 NZ_OU015946:498483-498824 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT241788 WP_004150912.1 NZ_CP095253:3103869-3104162 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT241788 NZ_OU015946:498253-498483 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References