Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4625331..4626106 Replicon chromosome
Accession NZ_CP068684
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76-pV

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JMY88_RS22765 Protein ID WP_004150910.1
Coordinates 4625331..4625816 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JMY88_RS22770 Protein ID WP_004150912.1
Coordinates 4625813..4626106 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY88_RS22740 4620500..4621867 - 1368 WP_004150905.1 replicative DNA helicase -
JMY88_RS22745 4621860..4622243 - 384 WP_004150906.1 hypothetical protein -
JMY88_RS22750 4622243..4623115 - 873 WP_004188557.1 ParA family protein -
JMY88_RS22755 4623721..4623937 - 217 Protein_4444 transposase -
JMY88_RS22760 4624034..4624627 - 594 WP_004188553.1 hypothetical protein -
JMY88_RS22765 4625331..4625816 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JMY88_RS22770 4625813..4626106 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JMY88_RS22775 4626751..4628127 + 1377 WP_004217775.1 cystathionine beta-synthase -
JMY88_RS22780 4628138..4629286 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JMY88_RS22785 4629287..4630198 - 912 WP_004188550.1 LysR family transcriptional regulator -
JMY88_RS22790 4630296..4630898 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4568281..4626357 58076
flank IS/Tn - - 4623785..4623937 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T187543 WP_004150910.1 NZ_CP068684:c4625816-4625331 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T187543 NZ_CP089884:335531-335899 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT187543 WP_004150912.1 NZ_CP068684:c4626106-4625813 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT187543 NZ_CP089884:335313-335534 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References