Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5234288..5235063 Replicon chromosome
Accession NZ_CP020901
Organism Klebsiella pneumoniae strain K66-45

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag B5G58_RS26140 Protein ID WP_004150910.1
Coordinates 5234578..5235063 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag B5G58_RS26135 Protein ID WP_004150912.1
Coordinates 5234288..5234581 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5G58_RS26115 5229496..5230098 - 603 WP_002916735.1 short chain dehydrogenase -
B5G58_RS26120 5230196..5231107 + 912 WP_004188550.1 LysR family transcriptional regulator -
B5G58_RS26125 5231108..5232256 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
B5G58_RS26130 5232267..5233643 - 1377 WP_004217775.1 cystathionine beta-synthase -
B5G58_RS26135 5234288..5234581 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
B5G58_RS26140 5234578..5235063 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
B5G58_RS26145 5235767..5236360 + 594 WP_004188553.1 hypothetical protein -
B5G58_RS26150 5236457..5236673 + 217 Protein_4992 transposase -
B5G58_RS26165 5237279..5238151 + 873 WP_004188557.1 ParA family protein -
B5G58_RS26170 5238151..5238534 + 384 WP_004150906.1 hypothetical protein -
B5G58_RS26175 5238527..5239894 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 5236457..5236609 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T75667 WP_004150910.1 NZ_CP020901:5234578-5235063 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T75667 NZ_CP020901:5234578-5235063 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT75667 WP_004150912.1 NZ_CP020901:5234288-5234581 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT75667 NZ_CP020901:5234288-5234581 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References