Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 691325..692100 Replicon chromosome
Accession NZ_CP072653
Organism Klebsiella pneumoniae strain KPN234

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag J8J76_RS03440 Protein ID WP_019704987.1
Coordinates 691615..692100 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J8J76_RS03435 Protein ID WP_004150912.1
Coordinates 691325..691618 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8J76_RS03415 (J8J76_03415) 686534..687136 - 603 WP_004174410.1 short chain dehydrogenase -
J8J76_RS03420 (J8J76_03420) 687234..688145 + 912 WP_069339825.1 LysR family transcriptional regulator -
J8J76_RS03425 (J8J76_03425) 688146..689294 - 1149 WP_087796222.1 PLP-dependent aspartate aminotransferase family protein -
J8J76_RS03430 (J8J76_03430) 689305..690681 - 1377 WP_004181304.1 cystathionine beta-synthase -
J8J76_RS03435 (J8J76_03435) 691325..691618 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J8J76_RS03440 (J8J76_03440) 691615..692100 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
J8J76_RS03445 (J8J76_03445) 692804..693397 + 594 WP_004188553.1 hypothetical protein -
J8J76_RS03450 (J8J76_03450) 693494..693710 + 217 Protein_678 transposase -
J8J76_RS03460 (J8J76_03460) 694551..695264 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 693494..693646 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T198204 WP_019704987.1 NZ_CP072653:691615-692100 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T198204 NZ_CP096843:712066-712467 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT198204 WP_004150912.1 NZ_CP072653:691325-691618 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT198204 NZ_CP096843:711842-712069 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References