Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 734724..735499 Replicon chromosome
Accession NZ_CP089509
Organism Klebsiella pneumoniae strain GD21SC417

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LT986_RS03645 Protein ID WP_004150910.1
Coordinates 735014..735499 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LT986_RS03640 Protein ID WP_004150912.1
Coordinates 734724..735017 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT986_RS03620 (729932) 729932..730534 - 603 WP_002916735.1 short chain dehydrogenase -
LT986_RS03625 (730632) 730632..731543 + 912 WP_105910473.1 LysR family transcriptional regulator -
LT986_RS03630 (731544) 731544..732692 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LT986_RS03635 (732703) 732703..734079 - 1377 WP_004217775.1 cystathionine beta-synthase -
LT986_RS03640 (734724) 734724..735017 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LT986_RS03645 (735014) 735014..735499 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LT986_RS03650 (736203) 736203..736796 + 594 WP_004188553.1 hypothetical protein -
LT986_RS03655 (736893) 736893..737109 + 217 Protein_719 transposase -
LT986_RS03665 (737787) 737787..738500 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 736893..737045 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T228328 WP_004150910.1 NZ_CP089509:735014-735499 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T228328 NZ_CP123767:42745-43065 [Acinetobacter pittii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT228328 WP_004150912.1 NZ_CP089509:734724-735017 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT228328 NZ_CP123767:43058-43330 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References