Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740781..741556 Replicon chromosome
Accession NZ_CP030300
Organism Klebsiella pneumoniae strain 283747

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag DRB02_RS03700 Protein ID WP_004150910.1
Coordinates 741071..741556 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag DRB02_RS03695 Protein ID WP_004150912.1
Coordinates 740781..741074 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB02_RS03675 (DRB02_03815) 735989..736591 - 603 WP_062954968.1 short chain dehydrogenase -
DRB02_RS03680 (DRB02_03820) 736689..737600 + 912 WP_004188550.1 LysR family transcriptional regulator -
DRB02_RS03685 (DRB02_03825) 737601..738749 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
DRB02_RS03690 (DRB02_03830) 738760..740136 - 1377 WP_004217775.1 cystathionine beta-synthase -
DRB02_RS03695 (DRB02_03835) 740781..741074 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
DRB02_RS03700 (DRB02_03840) 741071..741556 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
DRB02_RS03705 (DRB02_03845) 742260..742853 + 594 WP_004188553.1 hypothetical protein -
DRB02_RS03710 (DRB02_03850) 742950..743166 + 217 Protein_730 transposase -
DRB02_RS03715 (DRB02_03865) 743772..744644 + 873 WP_004188557.1 ParA family protein -
DRB02_RS03720 (DRB02_03870) 744644..745027 + 384 WP_004150906.1 hypothetical protein -
DRB02_RS03725 (DRB02_03875) 745020..746387 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742950..743102 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T107538 WP_004150910.1 NZ_CP030300:741071-741556 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T107538 NZ_CP030300:741071-741556 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT107538 WP_004150912.1 NZ_CP030300:740781-741074 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT107538 NZ_CP030300:740781-741074 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References