Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 715282..716057 Replicon chromosome
Accession NZ_CP125734
Organism Klebsiella pneumoniae strain GYB02

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag QMW18_RS03540 Protein ID WP_004889762.1
Coordinates 715572..716057 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag QMW18_RS03535 Protein ID WP_004150912.1
Coordinates 715282..715575 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMW18_RS03515 (QMW18_03515) 710491..711093 - 603 WP_004174410.1 short chain dehydrogenase -
QMW18_RS03520 (QMW18_03520) 711191..712102 + 912 WP_017879796.1 LysR family transcriptional regulator -
QMW18_RS03525 (QMW18_03525) 712103..713251 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
QMW18_RS03530 (QMW18_03530) 713262..714638 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
QMW18_RS03535 (QMW18_03535) 715282..715575 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
QMW18_RS03540 (QMW18_03540) 715572..716057 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
QMW18_RS03545 (QMW18_03545) 716761..717353 + 593 Protein_697 hypothetical protein -
QMW18_RS03550 (QMW18_03550) 717450..717666 + 217 Protein_698 transposase -
QMW18_RS03560 (QMW18_03560) 718181..718894 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 717450..717602 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T282318 WP_004889762.1 NZ_CP125734:715572-716057 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT282318 WP_004150912.1 NZ_CP125734:715282-715575 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References