Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741179..741954 Replicon chromosome
Accession NZ_CP045263
Organism Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag ELO34_RS03760 Protein ID WP_004150910.1
Coordinates 741469..741954 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ELO34_RS03755 Protein ID WP_004150912.1
Coordinates 741179..741472 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELO34_RS03735 736387..736989 - 603 WP_062954968.1 short chain dehydrogenase -
ELO34_RS03740 737087..737998 + 912 WP_004188550.1 LysR family transcriptional regulator -
ELO34_RS03745 737999..739147 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
ELO34_RS03750 739158..740534 - 1377 WP_004217775.1 cystathionine beta-synthase -
ELO34_RS03755 741179..741472 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ELO34_RS03760 741469..741954 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
ELO34_RS03765 742658..743251 + 594 WP_004188553.1 hypothetical protein -
ELO34_RS03770 743348..743564 + 217 Protein_729 transposase -
ELO34_RS03775 744170..745042 + 873 WP_004188557.1 ParA family protein -
ELO34_RS03780 745042..745425 + 384 WP_004150906.1 hypothetical protein -
ELO34_RS03785 745418..746785 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743348..743500 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T141582 WP_004150910.1 NZ_CP045263:741469-741954 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T141582 NZ_CP060018:3142400-3142732 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
GTGAACCGAGGAGAGATCTGGACCGTAGCGGGGGGTGTCTACTCGACAAAACCGCGTCCCGCGGTGATCGTCCAGGATGA
TTTGTTCGATGCCACGATGTCGGTGGCGGTTGCGCCGATCACCAGCGCATTACTGGAGGCTCCGCTAATGCGCATTCGGA
TAGCCGGCGGAGACGGACGGTTATCCGGACTCGACCACGACAGTGACGTGATGATCGACAAGCTCACAACTGTCAGGAGG
TCGAACGTCCACGCGCGAGTCGGTCGGCTGACTGCGGAACAAGTCGTCGAAGTCGAGCGAGCCATGATGGCATTTCTCGG
GCTTGCGCGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT141582 WP_004150912.1 NZ_CP045263:741179-741472 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT141582 NZ_CP060018:3142197-3142403 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
ATGGCGGTGAGAGACAGGGTCGGCGAGTACCGCCGGCGCATGCGGGAGCGCGGCCTGCGCCCACTGCAAGTCTGGGTGCC
TGACGTGCGCACCGAGAGTTTTGCCGCCGAGGCTCATCGTCAATCGTCACTGGTGGCGCGGGCGGATGTAAACAGCGATG
ATCAGGACTTCATCGAAGCTGTCTCGACGCCATGGGACGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References