Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743814..744589 Replicon chromosome
Accession NZ_CP055294
Organism Klebsiella pneumoniae strain KP55

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HUW33_RS03710 Protein ID WP_004150910.1
Coordinates 744104..744589 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HUW33_RS03705 Protein ID WP_004150912.1
Coordinates 743814..744107 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW33_RS03685 (HUW33_03685) 739022..739624 - 603 WP_062954968.1 short chain dehydrogenase -
HUW33_RS03690 (HUW33_03690) 739722..740633 + 912 WP_004188550.1 LysR family transcriptional regulator -
HUW33_RS03695 (HUW33_03695) 740634..741782 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HUW33_RS03700 (HUW33_03700) 741793..743169 - 1377 WP_004217775.1 cystathionine beta-synthase -
HUW33_RS03705 (HUW33_03705) 743814..744107 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HUW33_RS03710 (HUW33_03710) 744104..744589 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HUW33_RS03715 (HUW33_03715) 745293..745886 + 594 WP_004188553.1 hypothetical protein -
HUW33_RS03720 (HUW33_03720) 745983..746199 + 217 Protein_732 transposase -
HUW33_RS03725 (HUW33_03725) 746805..747677 + 873 WP_004188557.1 ParA family protein -
HUW33_RS03730 (HUW33_03730) 747677..748060 + 384 WP_004150906.1 hypothetical protein -
HUW33_RS03735 (HUW33_03735) 748053..749420 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 745983..746135 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T162283 WP_004150910.1 NZ_CP055294:744104-744589 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T162283 NZ_CP071711:c3697608-3697234 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT162283 WP_004150912.1 NZ_CP055294:743814-744107 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT162283 NZ_CP071711:c3698066-3697698 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References