Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3317363..3318138 Replicon chromosome
Accession NZ_CP107352
Organism Klebsiella pneumoniae strain CRKP_35

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag OH515_RS16025 Protein ID WP_019704987.1
Coordinates 3317653..3318138 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag OH515_RS16020 Protein ID WP_004150912.1
Coordinates 3317363..3317656 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH515_RS16000 (3312572) 3312572..3313174 - 603 WP_004174410.1 short chain dehydrogenase -
OH515_RS16005 (3313272) 3313272..3314183 + 912 WP_004181308.1 LysR family transcriptional regulator -
OH515_RS16010 (3314184) 3314184..3315332 - 1149 WP_134919031.1 PLP-dependent aspartate aminotransferase family protein -
OH515_RS16015 (3315343) 3315343..3316719 - 1377 WP_072158501.1 cystathionine beta-synthase -
OH515_RS16020 (3317363) 3317363..3317656 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
OH515_RS16025 (3317653) 3317653..3318138 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
OH515_RS16030 (3318842) 3318842..3319434 + 593 Protein_3122 hypothetical protein -
OH515_RS16035 (3319531) 3319531..3319747 + 217 Protein_3123 transposase -
OH515_RS16045 (3320425) 3320425..3321138 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3319531..3319683 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T260880 WP_019704987.1 NZ_CP107352:3317653-3318138 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T260880 NZ_CP107352:3317653-3318138 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTAATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT260880 WP_004150912.1 NZ_CP107352:3317363-3317656 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT260880 NZ_CP107352:3317363-3317656 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References