Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1817302..1818077 Replicon chromosome
Accession NZ_CP012883
Organism Klebsiella pneumoniae KP-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag KLP1_RS09025 Protein ID WP_021314147.1
Coordinates 1817302..1817787 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KLP1_RS09030 Protein ID WP_004150912.1
Coordinates 1817784..1818077 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLP1_RS09000 1814138..1814851 + 714 WP_002916694.1 DUF554 domain-containing protein -
KLP1_RS09015 1815692..1815908 - 217 Protein_1731 transposase -
KLP1_RS09020 1816005..1816598 - 594 WP_004188553.1 hypothetical protein -
KLP1_RS09025 1817302..1817787 - 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
KLP1_RS09030 1817784..1818077 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KLP1_RS09035 1818722..1820098 + 1377 WP_004217775.1 cystathionine beta-synthase -
KLP1_RS09040 1820109..1821257 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KLP1_RS09045 1821258..1822169 - 912 WP_004188550.1 LysR family transcriptional regulator -
KLP1_RS09050 1822267..1822869 + 603 WP_021440720.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1815756..1815908 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T57416 WP_021314147.1 NZ_CP012883:c1817787-1817302 [Klebsiella pneumoniae KP-1]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T57416 NZ_CP012883:c1817787-1817302 [Klebsiella pneumoniae KP-1]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT57416 WP_004150912.1 NZ_CP012883:c1818077-1817784 [Klebsiella pneumoniae KP-1]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT57416 NZ_CP012883:c1818077-1817784 [Klebsiella pneumoniae KP-1]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References