Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4108953..4109728 Replicon chromosome
Accession NZ_CP048430
Organism Klebsiella pneumoniae strain KP18-3-8

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GZ213_RS20865 Protein ID WP_004150910.1
Coordinates 4108953..4109438 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GZ213_RS20870 Protein ID WP_004150912.1
Coordinates 4109435..4109728 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ213_RS20840 4104122..4105489 - 1368 WP_004150905.1 replicative DNA helicase -
GZ213_RS20845 4105482..4105865 - 384 WP_004150906.1 hypothetical protein -
GZ213_RS20850 4105865..4106737 - 873 WP_004188557.1 ParA family protein -
GZ213_RS20855 4107343..4107559 - 217 Protein_4002 transposase -
GZ213_RS20860 4107656..4108249 - 594 WP_004188553.1 hypothetical protein -
GZ213_RS20865 4108953..4109438 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GZ213_RS20870 4109435..4109728 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GZ213_RS20875 4110373..4111749 + 1377 WP_004217775.1 cystathionine beta-synthase -
GZ213_RS20880 4111760..4112908 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GZ213_RS20885 4112909..4113820 - 912 WP_004188550.1 LysR family transcriptional regulator -
GZ213_RS20890 4113918..4114520 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4049956..4109979 60023
flank IS/Tn - - 4107407..4107559 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T149430 WP_004150910.1 NZ_CP048430:c4109438-4108953 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T149430 NZ_CP064096:c3120383-3120033 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT149430 WP_004150912.1 NZ_CP048430:c4109728-4109435 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT149430 NZ_CP064096:c3120669-3120388 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References