Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737957..738732 Replicon chromosome
Accession NZ_CP028806
Organism Klebsiella pneumoniae strain WCHKP7E2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag B7D49_RS06870 Protein ID WP_004150910.1
Coordinates 738247..738732 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag B7D49_RS06865 Protein ID WP_004150912.1
Coordinates 737957..738250 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D49_RS06845 733165..733767 - 603 WP_002916735.1 short chain dehydrogenase -
B7D49_RS06850 733865..734776 + 912 WP_004188550.1 LysR family transcriptional regulator -
B7D49_RS06855 734777..735925 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
B7D49_RS06860 735936..737306 - 1371 WP_004150913.1 cystathionine beta-synthase -
B7D49_RS06865 737957..738250 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
B7D49_RS06870 738247..738732 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
B7D49_RS06875 739436..740029 + 594 WP_004188553.1 hypothetical protein -
B7D49_RS06880 740126..740342 + 217 Protein_753 IS3 family transposase -
B7D49_RS06885 740490..740675 + 186 WP_085666571.1 hypothetical protein -
B7D49_RS06890 740617..740838 + 222 WP_004188555.1 hypothetical protein -
B7D49_RS06895 740948..741820 + 873 WP_004188557.1 ParA family protein -
B7D49_RS06900 741820..742203 + 384 WP_004150906.1 hypothetical protein -
B7D49_RS06905 742196..743563 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 740126..740278 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T104209 WP_004150910.1 NZ_CP028806:738247-738732 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T104209 NZ_CP028806:738247-738732 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT104209 WP_004150912.1 NZ_CP028806:737957-738250 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT104209 NZ_CP028806:737957-738250 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References