Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2546296..2547071 Replicon chromosome
Accession NZ_CP070087
Organism Klebsiella pneumoniae strain FDAARGOS_1328

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I6K58_RS14145 Protein ID WP_004150910.1
Coordinates 2546586..2547071 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I6K58_RS14140 Protein ID WP_004150912.1
Coordinates 2546296..2546589 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K58_RS14120 2541504..2542106 - 603 WP_002916735.1 short chain dehydrogenase -
I6K58_RS14125 2542204..2543115 + 912 WP_004188550.1 LysR family transcriptional regulator -
I6K58_RS14130 2543116..2544264 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I6K58_RS14135 2544275..2545651 - 1377 WP_004217775.1 cystathionine beta-synthase -
I6K58_RS14140 2546296..2546589 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I6K58_RS14145 2546586..2547071 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I6K58_RS14150 2547775..2548368 + 594 WP_004188553.1 hypothetical protein -
I6K58_RS14155 2548465..2548681 + 217 Protein_2408 transposase -
I6K58_RS14160 2549287..2550159 + 873 WP_004188557.1 ParA family protein -
I6K58_RS14165 2550159..2550542 + 384 WP_004150906.1 hypothetical protein -
I6K58_RS14170 2550535..2551902 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2548465..2548617 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T193016 WP_004150910.1 NZ_CP070087:2546586-2547071 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T193016 NZ_CP092647:c3544442-3544164 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT193016 WP_004150912.1 NZ_CP070087:2546296-2546589 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT193016 NZ_CP092647:c3544705-3544445 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References