Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743950..744725 Replicon chromosome
Accession NZ_CP087623
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH50_RS03715 Protein ID WP_004150910.1
Coordinates 744240..744725 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH50_RS03710 Protein ID WP_004150912.1
Coordinates 743950..744243 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH50_RS03690 (LMH50_03690) 739158..739760 - 603 WP_062954968.1 short chain dehydrogenase -
LMH50_RS03695 (LMH50_03695) 739858..740769 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH50_RS03700 (LMH50_03700) 740770..741918 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH50_RS03705 (LMH50_03705) 741929..743305 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH50_RS03710 (LMH50_03710) 743950..744243 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH50_RS03715 (LMH50_03715) 744240..744725 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH50_RS03720 (LMH50_03720) 745429..746022 + 594 WP_004188553.1 hypothetical protein -
LMH50_RS03725 (LMH50_03725) 746119..746335 + 217 Protein_732 transposase -
LMH50_RS03730 (LMH50_03730) 746941..747813 + 873 WP_004188557.1 ParA family protein -
LMH50_RS03735 (LMH50_03735) 747813..748196 + 384 WP_004150906.1 hypothetical protein -
LMH50_RS03740 (LMH50_03740) 748189..749556 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 746119..746271 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225704 WP_004150910.1 NZ_CP087623:744240-744725 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225704 NZ_CP121294:c2783530-2783300 [Escherichia coli O155:H21]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225704 WP_004150912.1 NZ_CP087623:743950-744243 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225704 NZ_CP121294:c2783303-2782944 [Escherichia coli O155:H21]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References