Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 715076..715851 Replicon chromosome
Accession NZ_CP072422
Organism Klebsiella pneumoniae strain B33769

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag J6255_RS03535 Protein ID WP_004889762.1
Coordinates 715366..715851 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J6255_RS03530 Protein ID WP_004150912.1
Coordinates 715076..715369 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6255_RS03510 (J6255_03510) 710285..710887 - 603 WP_004174410.1 short chain dehydrogenase -
J6255_RS03515 (J6255_03515) 710985..711896 + 912 WP_017879796.1 LysR family transcriptional regulator -
J6255_RS03520 (J6255_03520) 711897..713045 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
J6255_RS03525 (J6255_03525) 713056..714432 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
J6255_RS03530 (J6255_03530) 715076..715369 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J6255_RS03535 (J6255_03535) 715366..715851 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
J6255_RS03540 (J6255_03540) 716555..717147 + 593 Protein_697 hypothetical protein -
J6255_RS03545 (J6255_03545) 717244..717460 + 217 Protein_698 transposase -
J6255_RS03555 (J6255_03555) 717975..718688 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 717244..717396 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T197749 WP_004889762.1 NZ_CP072422:715366-715851 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T197749 NZ_CP096668:2309-2650 [Yersinia pestis EV76-CN]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT197749 WP_004150912.1 NZ_CP072422:715076-715369 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT197749 NZ_CP096668:2650-2949 [Yersinia pestis EV76-CN]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References