Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 729257..730032 Replicon chromosome
Accession NZ_CP061380
Organism Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HCV86_RS03610 Protein ID WP_004150910.1
Coordinates 729547..730032 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HCV86_RS03605 Protein ID WP_004150912.1
Coordinates 729257..729550 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV86_RS03585 724465..725067 - 603 WP_002916735.1 short chain dehydrogenase -
HCV86_RS03590 725165..726076 + 912 WP_004188550.1 LysR family transcriptional regulator -
HCV86_RS03595 726077..727225 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HCV86_RS03600 727236..728612 - 1377 WP_004217775.1 cystathionine beta-synthase -
HCV86_RS03605 729257..729550 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HCV86_RS03610 729547..730032 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HCV86_RS03615 730736..731329 + 594 WP_004188553.1 hypothetical protein -
HCV86_RS03620 731426..731642 + 217 Protein_711 transposase -
HCV86_RS03630 732320..733033 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 731426..731578 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T172533 WP_004150910.1 NZ_CP061380:729547-730032 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T172533 NZ_CP079780:c305006-304899 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT172533 WP_004150912.1 NZ_CP061380:729257-729550 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT172533 NZ_CP079780:305054-305120 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References