Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4532616..4533391 Replicon chromosome
Accession NZ_CP009872
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH30

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPNIH30_RS22835 Protein ID WP_004150910.1
Coordinates 4532616..4533101 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNIH30_RS22840 Protein ID WP_004150912.1
Coordinates 4533098..4533391 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH30_RS22810 4527785..4529152 - 1368 WP_004150905.1 replicative DNA helicase -
KPNIH30_RS22815 4529145..4529528 - 384 WP_004150906.1 hypothetical protein -
KPNIH30_RS22820 4529528..4530400 - 873 WP_004188557.1 ParA family protein -
KPNIH30_RS22825 4531006..4531222 - 217 Protein_4350 transposase -
KPNIH30_RS22830 4531319..4531912 - 594 WP_004188553.1 hypothetical protein -
KPNIH30_RS22835 4532616..4533101 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPNIH30_RS22840 4533098..4533391 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNIH30_RS22845 4534036..4535412 + 1377 WP_004217775.1 cystathionine beta-synthase -
KPNIH30_RS22850 4535423..4536571 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPNIH30_RS22855 4536572..4537483 - 912 WP_004188550.1 LysR family transcriptional regulator -
KPNIH30_RS22860 4537581..4538183 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4475595..4533642 58047
flank IS/Tn - - 4531070..4531222 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T50089 WP_004150910.1 NZ_CP009872:c4533101-4532616 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T50089 NZ_CP009872:c4533101-4532616 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT50089 WP_004150912.1 NZ_CP009872:c4533391-4533098 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT50089 NZ_CP009872:c4533391-4533098 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References