Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3129212..3129987 Replicon chromosome
Accession NZ_CP077763
Organism Klebsiella pneumoniae strain KP63

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KQQ01_RS15595 Protein ID WP_004150910.1
Coordinates 3129212..3129697 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KQQ01_RS15600 Protein ID WP_004150912.1
Coordinates 3129694..3129987 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ01_RS15570 3124381..3125748 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
KQQ01_RS15575 3125741..3126124 - 384 WP_004150906.1 hypothetical protein -
KQQ01_RS15580 3126124..3126996 - 873 WP_004188557.1 ParA family protein -
KQQ01_RS15585 3127602..3127818 - 217 Protein_3072 transposase -
KQQ01_RS15590 3127915..3128508 - 594 WP_004188553.1 hypothetical protein -
KQQ01_RS15595 3129212..3129697 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KQQ01_RS15600 3129694..3129987 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KQQ01_RS15605 3130333..3131030 + 698 WP_223174630.1 IS1 family transposase -
KQQ01_RS15610 3131409..3132785 + 1377 WP_004217775.1 cystathionine beta-synthase -
KQQ01_RS15615 3132796..3133944 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KQQ01_RS15620 3133945..3134856 - 912 WP_004188550.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3069791..3130238 60447
- inside IScluster/Tn - - 3127666..3131030 3364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T207762 WP_004150910.1 NZ_CP077763:c3129697-3129212 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T207762 NZ_CP103508:c783526-783149 [Escherichia coli O25b:H4-ST131]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT207762 WP_004150912.1 NZ_CP077763:c3129987-3129694 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT207762 NZ_CP103508:c783983-783615 [Escherichia coli O25b:H4-ST131]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References