Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4481641..4482416 Replicon chromosome
Accession NC_012731
Organism Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag KP1_RS21865 Protein ID WP_004889762.1
Coordinates 4481641..4482126 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KP1_RS21870 Protein ID WP_004150912.1
Coordinates 4482123..4482416 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP1_RS21845 4478640..4479353 + 714 WP_002916694.1 DUF554 domain-containing protein -
KP1_RS21855 4480031..4480247 - 217 Protein_4174 transposase -
KP1_RS21860 4480344..4480937 - 594 WP_004188553.1 hypothetical protein -
KP1_RS21865 4481641..4482126 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
KP1_RS21870 4482123..4482416 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KP1_RS21875 4483060..4484436 + 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
KP1_RS21880 4484447..4485595 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
KP1_RS21885 4485596..4486507 - 912 WP_014906929.1 LysR family transcriptional regulator -
KP1_RS21890 4486605..4487207 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4480095..4480247 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T23836 WP_004889762.1 NC_012731:c4482126-4481641 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T23836 NC_012731:c4482126-4481641 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT23836 WP_004150912.1 NC_012731:c4482416-4482123 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT23836 NC_012731:c4482416-4482123 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References