Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 744675..745450 Replicon chromosome
Accession NZ_CP087645
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01754

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH55_RS03725 Protein ID WP_004150910.1
Coordinates 744965..745450 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH55_RS03720 Protein ID WP_004150912.1
Coordinates 744675..744968 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH55_RS03700 (LMH55_03700) 739883..740485 - 603 WP_062954968.1 short chain dehydrogenase -
LMH55_RS03705 (LMH55_03705) 740583..741494 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH55_RS03710 (LMH55_03710) 741495..742643 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH55_RS03715 (LMH55_03715) 742654..744030 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH55_RS03720 (LMH55_03720) 744675..744968 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH55_RS03725 (LMH55_03725) 744965..745450 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH55_RS03730 (LMH55_03730) 746154..746747 + 594 WP_004188553.1 hypothetical protein -
LMH55_RS03735 (LMH55_03735) 746844..747060 + 217 Protein_734 transposase -
LMH55_RS03740 (LMH55_03740) 747666..748538 + 873 WP_004188557.1 ParA family protein -
LMH55_RS03745 (LMH55_03745) 748538..748921 + 384 WP_004150906.1 hypothetical protein -
LMH55_RS03750 (LMH55_03750) 748914..750281 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 746844..746996 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225809 WP_004150910.1 NZ_CP087645:744965-745450 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225809 NZ_CP121365:c110617-110354 [Acinetobacter baumannii]
ATGACGAGTCGTAATGTCGAATGGACTGAAAATGCCTGGGATGAATATATCTATTGGCAAACACAGGATAAAAAGATACT
CAAACGTATCAATACCTTAATCAGAGAATGTCAAAGAACACCCTTTGAAGGAACTGGGAAACCAGAACCTTTAAAAGCAA
ATCTTTCAGGATTCTGGAGCCGTAGAATTGATGAGAAGCATAGATTAGTTTATGAAGTGACGGATGAACGAATTTCCATT
ATTCAATGTCGGTTCCATTACTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225809 WP_004150912.1 NZ_CP087645:744675-744968 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225809 NZ_CP121365:c110867-110604 [Acinetobacter baumannii]
ATGCATGTATTTACATATACAGACGCACGTAACAACTTAAAATCTGTTTTAGATAAAGTTATTGATGATGCAGATGTCGC
TTTCATTACAAGAAAAGAAGGCGGTCATGCTGTAGTAATGGGACAAGATCATTACGATAGTTTAATGGAAACGTTGTATC
TCCTTTCATCTCCAAATAATACCACTCGTTTAAACGAATCAATTGCTCAATTACGCGCAGGTAAAACCAAACAAAGAGAG
TTGATTAAAGATGACGAGTCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References