Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2440893..2441668 Replicon chromosome
Accession NZ_CP053364
Organism Klebsiella pneumoniae strain BA2275

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag C5707_RS12155 Protein ID WP_004150910.1
Coordinates 2440893..2441378 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C5707_RS12160 Protein ID WP_004150912.1
Coordinates 2441375..2441668 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5707_RS12130 2436062..2437429 - 1368 WP_004150905.1 replicative DNA helicase -
C5707_RS12135 2437422..2437805 - 384 WP_004150906.1 hypothetical protein -
C5707_RS12140 2437805..2438677 - 873 WP_004188557.1 ParA family protein -
C5707_RS12145 2439284..2439500 - 217 Protein_2387 transposase -
C5707_RS12150 2439597..2440189 - 593 Protein_2388 hypothetical protein -
C5707_RS12155 2440893..2441378 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
C5707_RS12160 2441375..2441668 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C5707_RS12165 2442313..2443689 + 1377 WP_004217775.1 cystathionine beta-synthase -
C5707_RS12170 2443700..2444848 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
C5707_RS12175 2444849..2445760 - 912 WP_004188550.1 LysR family transcriptional regulator -
C5707_RS12180 2445858..2446460 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2383873..2441919 58046
flank IS/Tn - - 2439348..2439500 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T156701 WP_004150910.1 NZ_CP053364:c2441378-2440893 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T156701 NZ_CP069069:c2866736-2866317 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGACGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT156701 WP_004150912.1 NZ_CP053364:c2441668-2441375 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT156701 NZ_CP069069:c2866957-2866733 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References