Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 726555..727330 Replicon chromosome
Accession NZ_CP081889
Organism Klebsiella pneumoniae strain IA-044

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag KZ664_RS03605 Protein ID WP_009308645.1
Coordinates 726845..727330 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KZ664_RS03600 Protein ID WP_004150912.1
Coordinates 726555..726848 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ664_RS03580 (KZ664_03570) 721763..722365 - 603 WP_004174410.1 short chain dehydrogenase -
KZ664_RS03585 (KZ664_03575) 722463..723374 + 912 WP_023342207.1 LysR family transcriptional regulator -
KZ664_RS03590 (KZ664_03580) 723375..724523 - 1149 WP_004174416.1 PLP-dependent aspartate aminotransferase family protein -
KZ664_RS03595 (KZ664_03585) 724534..725910 - 1377 WP_074428894.1 cystathionine beta-synthase -
KZ664_RS03600 (KZ664_03590) 726555..726848 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KZ664_RS03605 (KZ664_03595) 726845..727330 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
KZ664_RS03610 (KZ664_03600) 728034..728627 + 594 WP_004188553.1 hypothetical protein -
KZ664_RS03615 (KZ664_03605) 728724..728940 + 217 Protein_710 transposase -
KZ664_RS03625 (KZ664_03615) 729618..730331 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 728724..728876 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T213641 WP_009308645.1 NZ_CP081889:726845-727330 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T213641 NZ_CP109745:c238694-238383 [Citrobacter portucalensis]
ATGCCACAGGTAATTGTCTCCGCACTGGCGCAGCGGGATTTACAGCGCCTGCAGGACTTTCTCAAAACCAAAAACCGGCT
GGCGGCCAGAAAAGCCGGTGAAGTGATCATCCGGGCTATCCAGCAGCTGAAAACGCAGCCTGACATGGGCCGCCCGGTTT
CTCTTCTCCCACTGGAATACCAGGAACTGGTGATCGGATTTGGCGACAGTGGTTATGTGATGCTCTATCGCCACGACCGG
GAAACGGACCGGATAGTGATCCTGACTGTCCGGCACCAGAAAGAAGCCGGCTATCACGGCCTGCAGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT213641 WP_004150912.1 NZ_CP081889:726555-726848 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT213641 NZ_CP109745:c238960-238682 [Citrobacter portucalensis]
ATGTCAGCTGCGACATCCATCAGGCTTGATGATGAACTCAAAGACCGCCTGAAAACCCTTGCCGATGACCGCCATCGCTC
CGCTCATGCCCTGATGCTGGAAGCGATCACCGAATACATCGACCGGGAAGAAAAGCGCAGCCAGTACCTGAGGGATGGAC
AGGCCGCATGGCAGCACTATCAGGAAACCGGTCTGCACCTGAGCGCCGAAGAGGTGGAAGCGTGGATAGATACCTGGGGA
ACTGAGAATGAGCAGGATGCTCCACCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References