Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742350..743125 Replicon chromosome
Accession NZ_CP027068
Organism Klebsiella pneumoniae strain WCHKP8F4

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag B7D38_RS04950 Protein ID WP_004150910.1
Coordinates 742640..743125 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag B7D38_RS04945 Protein ID WP_004150912.1
Coordinates 742350..742643 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D38_RS04925 737558..738160 - 603 WP_062954968.1 short chain dehydrogenase -
B7D38_RS04930 738258..739169 + 912 WP_004188550.1 LysR family transcriptional regulator -
B7D38_RS04935 739170..740318 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
B7D38_RS04940 740329..741699 - 1371 WP_004150913.1 cystathionine beta-synthase -
B7D38_RS04945 742350..742643 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
B7D38_RS04950 742640..743125 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
B7D38_RS04955 743829..744422 + 594 WP_004188553.1 hypothetical protein -
B7D38_RS04960 744519..744735 + 217 Protein_757 IS3 family transposase -
B7D38_RS04965 744883..745068 + 186 WP_085666571.1 hypothetical protein -
B7D38_RS04970 745010..745231 + 222 WP_004188555.1 hypothetical protein -
B7D38_RS04975 745341..746213 + 873 WP_004188557.1 ParA family protein -
B7D38_RS04980 746213..746596 + 384 WP_004150906.1 hypothetical protein -
B7D38_RS04985 746589..747956 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744519..744671 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T97223 WP_004150910.1 NZ_CP027068:742640-743125 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T97223 NZ_CP027068:742640-743125 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT97223 WP_004150912.1 NZ_CP027068:742350-742643 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT97223 NZ_CP027068:742350-742643 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References