Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4751785..4752560 Replicon chromosome
Accession NZ_CP025456
Organism Klebsiella pneumoniae strain KP69

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CYD98_RS24145 Protein ID WP_004150910.1
Coordinates 4751785..4752270 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CYD98_RS24150 Protein ID WP_004150912.1
Coordinates 4752267..4752560 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYD98_RS24115 4746954..4748321 - 1368 WP_004150905.1 replicative DNA helicase -
CYD98_RS24120 4748314..4748697 - 384 WP_004150906.1 hypothetical protein -
CYD98_RS24125 4748697..4749569 - 873 WP_004188557.1 ParA family protein -
CYD98_RS24135 4750175..4750391 - 217 Protein_4560 transposase -
CYD98_RS24140 4750488..4751081 - 594 WP_004188553.1 hypothetical protein -
CYD98_RS24145 4751785..4752270 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CYD98_RS24150 4752267..4752560 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CYD98_RS24155 4753205..4754581 + 1377 WP_004217775.1 cystathionine beta-synthase -
CYD98_RS24160 4754592..4755740 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CYD98_RS24165 4755741..4756652 - 912 WP_004188550.1 LysR family transcriptional regulator -
CYD98_RS24170 4756750..4757352 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4694765..4752811 58046
flank IS/Tn - - 4750239..4750391 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T90350 WP_004150910.1 NZ_CP025456:c4752270-4751785 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T90350 NZ_CP025456:c4752270-4751785 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT90350 WP_004150912.1 NZ_CP025456:c4752560-4752267 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT90350 NZ_CP025456:c4752560-4752267 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References