Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5130656..5131431 Replicon chromosome
Accession NZ_CP040711
Organism Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FGL96_RS25205 Protein ID WP_004150910.1
Coordinates 5130656..5131141 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FGL96_RS25210 Protein ID WP_004150912.1
Coordinates 5131138..5131431 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL96_RS25180 (FGL96_25350) 5125825..5127192 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
FGL96_RS25185 (FGL96_25355) 5127185..5127568 - 384 WP_004150906.1 hypothetical protein -
FGL96_RS25190 (FGL96_25360) 5127568..5128440 - 873 WP_004188557.1 ParA family protein -
FGL96_RS25195 (FGL96_25375) 5129046..5129262 - 217 Protein_4927 transposase -
FGL96_RS25200 (FGL96_25380) 5129359..5129952 - 594 WP_004188553.1 hypothetical protein -
FGL96_RS25205 (FGL96_25385) 5130656..5131141 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FGL96_RS25210 (FGL96_25390) 5131138..5131431 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FGL96_RS25215 (FGL96_25395) 5132076..5133452 + 1377 WP_004217775.1 cystathionine beta-synthase -
FGL96_RS25220 (FGL96_25400) 5133463..5134611 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FGL96_RS25225 (FGL96_25405) 5134612..5135523 - 912 WP_004188550.1 LysR family transcriptional regulator -
FGL96_RS25230 (FGL96_25410) 5135621..5136223 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 5073668..5131682 58014
flank IS/Tn - - 5129110..5129262 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T126884 WP_004150910.1 NZ_CP040711:c5131141-5130656 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T126884 NZ_CP040711:c5131141-5130656 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT126884 WP_004150912.1 NZ_CP040711:c5131431-5131138 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT126884 NZ_CP040711:c5131431-5131138 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References