Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 731432..732207 Replicon chromosome
Accession NZ_CP043580
Organism Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag C6M74_RS03620 Protein ID WP_004889762.1
Coordinates 731722..732207 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C6M74_RS03615 Protein ID WP_004150912.1
Coordinates 731432..731725 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M74_RS03595 (726641) 726641..727243 - 603 WP_002916735.1 short chain dehydrogenase -
C6M74_RS03600 (727341) 727341..728252 + 912 WP_014906929.1 LysR family transcriptional regulator -
C6M74_RS03605 (728253) 728253..729401 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
C6M74_RS03610 (729412) 729412..730788 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
C6M74_RS03615 (731432) 731432..731725 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C6M74_RS03620 (731722) 731722..732207 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
C6M74_RS03625 (732911) 732911..733504 + 594 WP_004188553.1 hypothetical protein -
C6M74_RS03630 (733601) 733601..733817 + 217 Protein_714 transposase -
C6M74_RS03640 (734495) 734495..735208 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 733601..733753 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T139054 WP_004889762.1 NZ_CP043580:731722-732207 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T139054 NZ_CP058343:c104942-104784 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT139054 WP_004150912.1 NZ_CP043580:731432-731725 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT139054 NZ_CP058343:c105210-104986 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References