Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 734653..735428 Replicon chromosome
Accession NZ_CP068015
Organism Klebsiella pneumoniae strain 2016_49

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag JKF56_RS03625 Protein ID WP_004889762.1
Coordinates 734943..735428 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JKF56_RS03620 Protein ID WP_004150912.1
Coordinates 734653..734946 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF56_RS03600 (729862) 729862..730464 - 603 WP_002916735.1 short chain dehydrogenase -
JKF56_RS03605 (730562) 730562..731473 + 912 WP_014906929.1 LysR family transcriptional regulator -
JKF56_RS03610 (731474) 731474..732622 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
JKF56_RS03615 (732633) 732633..734009 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
JKF56_RS03620 (734653) 734653..734946 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JKF56_RS03625 (734943) 734943..735428 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
JKF56_RS03630 (736132) 736132..736725 + 594 WP_004188553.1 hypothetical protein -
JKF56_RS03635 (736822) 736822..737038 + 217 Protein_716 transposase -
JKF56_RS03645 (737716) 737716..738429 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 736822..736974 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T186417 WP_004889762.1 NZ_CP068015:734943-735428 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T186417 NZ_CP089428:153714-154001 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT186417 WP_004150912.1 NZ_CP068015:734653-734946 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT186417 NZ_CP089428:153475-153714 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References