Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736099..736874 Replicon chromosome
Accession NZ_CP066517
Organism Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag H3G99_RS03675 Protein ID WP_004150910.1
Coordinates 736389..736874 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag H3G99_RS03670 Protein ID WP_004150912.1
Coordinates 736099..736392 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G99_RS03650 731307..731909 - 603 WP_062954968.1 short chain dehydrogenase -
H3G99_RS03655 732007..732918 + 912 WP_004188550.1 LysR family transcriptional regulator -
H3G99_RS03660 732919..734067 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
H3G99_RS03665 734078..735454 - 1377 WP_004217775.1 cystathionine beta-synthase -
H3G99_RS03670 736099..736392 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
H3G99_RS03675 736389..736874 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
H3G99_RS03680 737578..738171 + 594 WP_004188553.1 hypothetical protein -
H3G99_RS03685 738268..738484 + 217 Protein_724 transposase -
H3G99_RS03690 739090..739962 + 873 WP_004188557.1 ParA family protein -
H3G99_RS03695 739962..740345 + 384 WP_004150906.1 hypothetical protein -
H3G99_RS03700 740338..741705 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 738268..738420 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T184502 WP_004150910.1 NZ_CP066517:736389-736874 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T184502 NZ_CP087674:c1605147-1604965 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAACTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT184502 WP_004150912.1 NZ_CP066517:736099-736392 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT184502 NZ_CP087674:c1604918-1604436 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGTGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATCCCCGCAGCCAGCCAGGCGCAACCAGGCGAGCGCGTGATCCATCTGCCGGCGGTTACAGTTGCG
AAGATCGCGCTCTGGAACGAAATGGTCCGGCGAGATATGCGAAAGGCCGACCTCTGCAGGCTGCTCGGTATCGCACAAAC
TCAGGGCGACAGGCTCGTTGACTTCCTCCACAACACCAAAATGGAAGCGCTGGAAAATGCGCTTGATTTTCTCGGCCTTC
GGCTGCGAGTTCAGATCGAGCGGGACAAAGACGTAGAGCGAAAAATGGCGGAACCGCCGGAGCGTGGAAAGCTCAGGGCC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References