Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 495627..496402 Replicon chromosome
Accession NZ_CP091978
Organism Klebsiella pneumoniae isolate JNQH373

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag L6V94_RS02435 Protein ID WP_004889762.1
Coordinates 495627..496112 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag L6V94_RS02440 Protein ID WP_004150912.1
Coordinates 496109..496402 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V94_RS02415 (492626) 492626..493339 + 714 WP_002916694.1 DUF554 domain-containing protein -
L6V94_RS02425 (494017) 494017..494233 - 217 Protein_469 transposase -
L6V94_RS02430 (494330) 494330..494923 - 594 WP_004188553.1 hypothetical protein -
L6V94_RS02435 (495627) 495627..496112 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
L6V94_RS02440 (496109) 496109..496402 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
L6V94_RS02445 (497046) 497046..498422 + 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
L6V94_RS02450 (498433) 498433..499581 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
L6V94_RS02455 (499582) 499582..500493 - 912 WP_014906929.1 LysR family transcriptional regulator -
L6V94_RS02460 (500591) 500591..501193 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 494081..494233 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T235324 WP_004889762.1 NZ_CP091978:c496112-495627 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T235324 NZ_LR134237:4299428-4299580 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT235324 WP_004150912.1 NZ_CP091978:c496402-496109 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT235324 NZ_LR134237:c4299379-4299322 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References