Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 746456..747231 Replicon chromosome
Accession NZ_CP090432
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LZ608_RS03730 Protein ID WP_004150910.1
Coordinates 746746..747231 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LZ608_RS03725 Protein ID WP_004150912.1
Coordinates 746456..746749 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ608_RS03705 (LZ608_03705) 741664..742266 - 603 WP_062954968.1 short chain dehydrogenase -
LZ608_RS03710 (LZ608_03710) 742364..743275 + 912 WP_004188550.1 LysR family transcriptional regulator -
LZ608_RS03715 (LZ608_03715) 743276..744424 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LZ608_RS03720 (LZ608_03720) 744435..745811 - 1377 WP_004217775.1 cystathionine beta-synthase -
LZ608_RS03725 (LZ608_03725) 746456..746749 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LZ608_RS03730 (LZ608_03730) 746746..747231 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LZ608_RS03735 (LZ608_03735) 747935..748528 + 594 WP_004188553.1 hypothetical protein -
LZ608_RS03740 (LZ608_03740) 748625..748841 + 217 Protein_735 transposase -
LZ608_RS03745 (LZ608_03745) 749447..750319 + 873 WP_004188557.1 ParA family protein -
LZ608_RS03750 (LZ608_03750) 750319..750702 + 384 WP_004150906.1 hypothetical protein -
LZ608_RS03755 (LZ608_03755) 750695..752062 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 748625..748777 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T230729 WP_004150910.1 NZ_CP090432:746746-747231 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T230729 NZ_CP125345:c1298-1032 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT230729 WP_004150912.1 NZ_CP090432:746456-746749 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT230729 NZ_CP125345:c1506-1282 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References