Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 812053..812828 Replicon chromosome
Accession NZ_CP082212
Organism Klebsiella pneumoniae strain BJ20

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6039_RS04190 Protein ID WP_004150910.1
Coordinates 812343..812828 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6039_RS04185 Protein ID WP_004150912.1
Coordinates 812053..812346 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6039_RS04165 (K6039_04140) 807261..807863 - 603 WP_062954968.1 short chain dehydrogenase -
K6039_RS04170 (K6039_04145) 807961..808872 + 912 WP_004188550.1 LysR family transcriptional regulator -
K6039_RS04175 (K6039_04150) 808873..810021 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6039_RS04180 (K6039_04155) 810032..811408 - 1377 WP_004217775.1 cystathionine beta-synthase -
K6039_RS04185 (K6039_04160) 812053..812346 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6039_RS04190 (K6039_04165) 812343..812828 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6039_RS04195 (K6039_04170) 813532..814125 + 594 WP_004188553.1 hypothetical protein -
K6039_RS04200 (K6039_04175) 814222..814438 + 217 Protein_826 transposase -
K6039_RS04205 (K6039_04180) 815044..815916 + 873 WP_004188557.1 ParA family protein -
K6039_RS04210 (K6039_04185) 815916..816299 + 384 WP_004150906.1 hypothetical protein -
K6039_RS04215 (K6039_04190) 816292..817659 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 814222..814374 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T214353 WP_004150910.1 NZ_CP082212:812343-812828 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T214353 NZ_CP110050:2815482-2815625 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAAGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT214353 WP_004150912.1 NZ_CP082212:812053-812346 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT214353 NZ_CP110050:c2815819-2815557 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References