Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4450542..4451317 Replicon chromosome
Accession NZ_CP065444
Organism Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5M62_RS22025 Protein ID WP_004150910.1
Coordinates 4450542..4451027 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5M62_RS22030 Protein ID WP_004150912.1
Coordinates 4451024..4451317 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M62_RS22000 4445711..4447078 - 1368 WP_004150905.1 replicative DNA helicase -
I5M62_RS22005 4447071..4447454 - 384 WP_004150906.1 hypothetical protein -
I5M62_RS22010 4447454..4448326 - 873 WP_004188557.1 ParA family protein -
I5M62_RS22015 4448932..4449148 - 217 Protein_4314 transposase -
I5M62_RS22020 4449245..4449838 - 594 WP_004188553.1 hypothetical protein -
I5M62_RS22025 4450542..4451027 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5M62_RS22030 4451024..4451317 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5M62_RS22035 4451962..4453338 + 1377 WP_004217775.1 cystathionine beta-synthase -
I5M62_RS22040 4453349..4454497 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5M62_RS22045 4454498..4455409 - 912 WP_004188550.1 LysR family transcriptional regulator -
I5M62_RS22050 4455507..4456109 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4392216..4451568 59352
flank IS/Tn - - 4448996..4449148 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182693 WP_004150910.1 NZ_CP065444:c4451027-4450542 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182693 NZ_CP086538:3885233-3885385 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182693 WP_004150912.1 NZ_CP065444:c4451317-4451024 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182693 NZ_CP086538:c3885184-3885127 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References