Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737265..738040 Replicon chromosome
Accession NZ_CP074541
Organism Klebsiella pneumoniae strain S24_CRE24

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KHV99_RS03690 Protein ID WP_004150910.1
Coordinates 737555..738040 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KHV99_RS03685 Protein ID WP_004150912.1
Coordinates 737265..737558 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV99_RS03665 (KHV99_03660) 732473..733075 - 603 WP_002916735.1 short chain dehydrogenase -
KHV99_RS03670 (KHV99_03665) 733173..734084 + 912 WP_004188550.1 LysR family transcriptional regulator -
KHV99_RS03675 (KHV99_03670) 734085..735233 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KHV99_RS03680 (KHV99_03675) 735244..736620 - 1377 WP_004217775.1 cystathionine beta-synthase -
KHV99_RS03685 (KHV99_03680) 737265..737558 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KHV99_RS03690 (KHV99_03685) 737555..738040 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KHV99_RS03695 (KHV99_03690) 738744..739337 + 594 WP_004188553.1 hypothetical protein -
KHV99_RS03700 (KHV99_03695) 739434..739650 + 217 Protein_727 transposase -
KHV99_RS03705 (KHV99_03700) 740256..741128 + 873 WP_004188557.1 ParA family protein -
KHV99_RS03710 (KHV99_03705) 741128..741511 + 384 WP_004150906.1 hypothetical protein -
KHV99_RS03715 (KHV99_03710) 741504..742871 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739434..739586 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T202320 WP_004150910.1 NZ_CP074541:737555-738040 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T202320 NZ_CP099374:c4293037-4292750 [Citrobacter braakii]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCTGAGGAAACATGGTGTTCGCTTTGAGGACGCAAT
ATTGGTTTTCGACGATCCCCAACATCTGTCGCGGCAGGAGCGGTATGAGAACGGTGAGTATCGCTGGCAGACCATTGGTC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGC
GCCAGAAAGGCAGATCGTAAAGAGAGGAATCGTTATGAGCATAGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT202320 WP_004150912.1 NZ_CP074541:737265-737558 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT202320 NZ_CP099374:c4292763-4292461 [Citrobacter braakii]
ATGAGCATAGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGCGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCATTCGCGGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCGTCGGTGCGCATTGATGCCGATGTGATGGAATGGCTGAAGCGCCCCGGGAAGGGT
TACCAGACGCGTCTCAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References