Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737584..738359 Replicon chromosome
Accession NZ_LR130548
Organism Klebsiella pneumoniae strain KPC2 isolate KPC2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag EW042_RS03750 Protein ID WP_004150910.1
Coordinates 737874..738359 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag EW042_RS03745 Protein ID WP_004150912.1
Coordinates 737584..737877 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW042_RS03725 732792..733394 - 603 WP_002916735.1 short chain dehydrogenase -
EW042_RS03730 733492..734403 + 912 WP_004188550.1 LysR family transcriptional regulator -
EW042_RS03735 734404..735552 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
EW042_RS03740 735563..736939 - 1377 WP_004217775.1 cystathionine beta-synthase -
EW042_RS03745 737584..737877 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
EW042_RS03750 737874..738359 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
EW042_RS03755 739063..739656 + 594 WP_004188553.1 hypothetical protein -
EW042_RS03760 739753..739969 + 217 Protein_725 transposase -
EW042_RS03775 740575..741447 + 873 WP_004188557.1 ParA family protein -
EW042_RS03780 741447..741830 + 384 WP_004150906.1 hypothetical protein -
EW042_RS03785 741823..743190 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739753..739905 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T286382 WP_004150910.1 NZ_LR130548:737874-738359 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT286382 WP_004150912.1 NZ_LR130548:737584-737877 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References