Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1095171..1095946 Replicon chromosome
Accession NZ_CP097419
Organism Klebsiella pneumoniae strain K229

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag M8Y66_RS05195 Protein ID WP_004150910.1
Coordinates 1095171..1095656 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag M8Y66_RS05200 Protein ID WP_004150912.1
Coordinates 1095653..1095946 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y66_RS05170 (M8Y66_05170) 1090340..1091707 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
M8Y66_RS05175 (M8Y66_05175) 1091700..1092083 - 384 WP_004150906.1 hypothetical protein -
M8Y66_RS05180 (M8Y66_05180) 1092083..1092955 - 873 WP_004188557.1 ParA family protein -
M8Y66_RS05185 (M8Y66_05185) 1093561..1093777 - 217 Protein_1011 transposase -
M8Y66_RS05190 (M8Y66_05190) 1093874..1094467 - 594 WP_004188553.1 hypothetical protein -
M8Y66_RS05195 (M8Y66_05195) 1095171..1095656 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
M8Y66_RS05200 (M8Y66_05200) 1095653..1095946 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
M8Y66_RS05205 (M8Y66_05205) 1096591..1097967 + 1377 WP_004217775.1 cystathionine beta-synthase -
M8Y66_RS05210 (M8Y66_05210) 1097978..1099126 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
M8Y66_RS05215 (M8Y66_05215) 1099127..1100038 - 912 WP_004188550.1 LysR family transcriptional regulator -
M8Y66_RS05220 (M8Y66_05220) 1100136..1100738 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 1036843..1096197 59354
flank IS/Tn - - 1093625..1093777 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T245409 WP_004150910.1 NZ_CP097419:c1095656-1095171 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT245409 WP_004150912.1 NZ_CP097419:c1095946-1095653 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References