Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3420313..3421088 Replicon chromosome
Accession NZ_CP063908
Organism Klebsiella pneumoniae strain M212-2

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag ISA82_RS16420 Protein ID WP_004889762.1
Coordinates 3420313..3420798 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISA82_RS16425 Protein ID WP_004150912.1
Coordinates 3420795..3421088 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA82_RS16400 3417312..3418025 + 714 WP_002916694.1 DUF554 domain-containing protein -
ISA82_RS16410 3418703..3418919 - 217 Protein_3216 transposase -
ISA82_RS16415 3419016..3419609 - 594 WP_004188553.1 hypothetical protein -
ISA82_RS16420 3420313..3420798 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
ISA82_RS16425 3420795..3421088 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISA82_RS16430 3421732..3423108 + 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
ISA82_RS16435 3423119..3424267 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
ISA82_RS16440 3424268..3425179 - 912 WP_014906929.1 LysR family transcriptional regulator -
ISA82_RS16445 3425277..3425879 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3418767..3418919 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T179370 WP_004889762.1 NZ_CP063908:c3420798-3420313 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179370 NZ_CP084713:c5731669-5731322 [Mycolicibacterium phocaicum]
ATGACCTCGGCTGAGCCACACCGCGGTGACTTATGGCTGGTCGCACTCGGTGCCGGTCGGGCCGGAGAACCGACGAAACA
CCGGCCCGCCGTTGTGCTGTCTGTTGATGATCTGCTCACCGGTGAACCCACCGAACTTGTCGTGGTGGTTCCCGTCTCCA
GCTCGCGAATCGCCACACCTCTGCGGCCCACAATCCGGCCGGGCGAGGGCGTCGAGGTGGACAGCGTCGCCGTCTGCCGC
GCCGTCCGTGGCATCGCCCGGGGTCGGCTGGTACGTCGGCTCGGGGTGCTCGCGGCACCAACGATGGGACAGATAGAGAA
ATCCCTGGCCCTGATCCTCGGTATATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179370 WP_004150912.1 NZ_CP063908:c3421088-3420795 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179370 NZ_CP084713:c5731892-5731656 [Mycolicibacterium phocaicum]
ATGGCGACACCGACGACGACAATCCGGGTCCCGGTCGCGACCCGTGACCGCCTCAACGCACGAGCTCGCGACCACGGTAT
TTCCGTTGTCGCGTTGCTCGACGAATGGGCCAACCGCGCAGAACGCGAGGATGCGTTTGCAGCGGAGCGGGCTGCCACCG
TGGCCGAGGCGACCAACGAAGCCGTCATCGAAGAGGACCAGGACTGGGGCCAGACGGACACCGATGACCTCGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References