Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4295318..4296093 Replicon chromosome
Accession NZ_CP102687
Organism Klebsiella pneumoniae strain GN4539

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NV950_RS21165 Protein ID WP_004150910.1
Coordinates 4295608..4296093 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NV950_RS21160 Protein ID WP_004150912.1
Coordinates 4295318..4295611 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV950_RS21140 (NV950_21135) 4290526..4291128 - 603 WP_002916735.1 short chain dehydrogenase -
NV950_RS21145 (NV950_21140) 4291226..4292137 + 912 WP_004188550.1 LysR family transcriptional regulator -
NV950_RS21150 (NV950_21145) 4292138..4293286 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NV950_RS21155 (NV950_21150) 4293297..4294673 - 1377 WP_004217775.1 cystathionine beta-synthase -
NV950_RS21160 (NV950_21155) 4295318..4295611 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NV950_RS21165 (NV950_21160) 4295608..4296093 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NV950_RS21170 (NV950_21165) 4296797..4297390 + 594 WP_004188553.1 hypothetical protein -
NV950_RS21175 (NV950_21170) 4297487..4297703 + 217 Protein_4136 transposase -
NV950_RS21180 (NV950_21175) 4298309..4299181 + 873 WP_004188557.1 ParA family protein -
NV950_RS21185 (NV950_21180) 4299181..4299564 + 384 WP_004150906.1 hypothetical protein -
NV950_RS21190 (NV950_21185) 4299557..4300924 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4297487..4297639 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T254012 WP_004150910.1 NZ_CP102687:4295608-4296093 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT254012 WP_004150912.1 NZ_CP102687:4295318-4295611 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References