Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 745479..746254 Replicon chromosome
Accession NZ_CP046957
Organism Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag C6M24_RS03740 Protein ID WP_004150910.1
Coordinates 745769..746254 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C6M24_RS03735 Protein ID WP_004150912.1
Coordinates 745479..745772 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M24_RS03715 (C6M24_03675) 740687..741289 - 603 WP_062954968.1 short chain dehydrogenase -
C6M24_RS03720 (C6M24_03680) 741387..742298 + 912 WP_004188550.1 LysR family transcriptional regulator -
C6M24_RS03725 (C6M24_03685) 742299..743447 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
C6M24_RS03730 (C6M24_03690) 743458..744834 - 1377 WP_004217775.1 cystathionine beta-synthase -
C6M24_RS03735 (C6M24_03695) 745479..745772 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C6M24_RS03740 (C6M24_03700) 745769..746254 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
C6M24_RS03745 (C6M24_03705) 746958..747551 + 594 WP_004188553.1 hypothetical protein -
C6M24_RS03750 (C6M24_03710) 747648..747864 + 217 Protein_737 transposase -
C6M24_RS03755 (C6M24_03725) 748470..749342 + 873 WP_004188557.1 ParA family protein -
C6M24_RS03760 (C6M24_03730) 749342..749725 + 384 WP_004150906.1 hypothetical protein -
C6M24_RS03765 (C6M24_03735) 749718..751085 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 747648..747800 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T145199 WP_004150910.1 NZ_CP046957:745769-746254 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T145199 NZ_CP062334:c91693-91229 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCGCACAATTACGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGTACTC
ACTACGGCGAATTCTCAATTACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCATATTGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT145199 WP_004150912.1 NZ_CP046957:745479-745772 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT145199 NZ_CP062334:c92020-91706 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATTTA
GAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References