Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742223..742998 Replicon chromosome
Accession NZ_CP043344
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CVC44_RS03700 Protein ID WP_004150910.1
Coordinates 742513..742998 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CVC44_RS03695 Protein ID WP_004150912.1
Coordinates 742223..742516 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVC44_RS03675 (CVC44_03690) 737431..738033 - 603 WP_062954968.1 short chain dehydrogenase -
CVC44_RS03680 (CVC44_03695) 738131..739042 + 912 WP_004188550.1 LysR family transcriptional regulator -
CVC44_RS03685 (CVC44_03700) 739043..740191 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CVC44_RS03690 (CVC44_03705) 740202..741578 - 1377 WP_004217775.1 cystathionine beta-synthase -
CVC44_RS03695 (CVC44_03710) 742223..742516 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CVC44_RS03700 (CVC44_03715) 742513..742998 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CVC44_RS03705 (CVC44_03720) 743702..744295 + 594 WP_004188553.1 hypothetical protein -
CVC44_RS03710 (CVC44_03725) 744392..744608 + 217 Protein_730 transposase -
CVC44_RS03715 (CVC44_03740) 745214..746086 + 873 WP_004188557.1 ParA family protein -
CVC44_RS03720 (CVC44_03745) 746086..746469 + 384 WP_004150906.1 hypothetical protein -
CVC44_RS03725 (CVC44_03750) 746462..747829 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744392..744544 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T138423 WP_004150910.1 NZ_CP043344:742513-742998 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T138423 NZ_CP057722:c3710722-3710405 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT138423 WP_004150912.1 NZ_CP043344:742223-742516 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT138423 NZ_CP057722:c3710412-3710125 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References