Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738150..738925 Replicon chromosome
Accession NZ_CP092589
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MKT41_RS03690 Protein ID WP_004150910.1
Coordinates 738440..738925 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MKT41_RS03685 Protein ID WP_004150912.1
Coordinates 738150..738443 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS03665 (MKT41_03665) 733358..733960 - 603 WP_002916735.1 short chain dehydrogenase -
MKT41_RS03670 (MKT41_03670) 734058..734969 + 912 WP_004188550.1 LysR family transcriptional regulator -
MKT41_RS03675 (MKT41_03675) 734970..736118 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MKT41_RS03680 (MKT41_03680) 736129..737505 - 1377 WP_004217775.1 cystathionine beta-synthase -
MKT41_RS03685 (MKT41_03685) 738150..738443 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MKT41_RS03690 (MKT41_03690) 738440..738925 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MKT41_RS03695 (MKT41_03695) 739629..740222 + 594 WP_004188553.1 hypothetical protein -
MKT41_RS03700 (MKT41_03700) 740319..740535 + 217 Protein_727 transposase -
MKT41_RS03705 (MKT41_03705) 741335..742195 + 861 WP_000158645.1 DUF6387 family protein -
MKT41_RS03710 (MKT41_03710) 742411..742659 + 249 WP_000616796.1 AlpA family transcriptional regulator -
MKT41_RS03715 (MKT41_03715) 742656..742967 + 312 WP_000837631.1 hypothetical protein -
MKT41_RS03720 (MKT41_03720) 742982..743368 + 387 WP_001545719.1 hypothetical protein -
MKT41_RS03725 (MKT41_03725) 743385..743672 + 288 WP_001291073.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 740319..740471 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T236712 WP_004150910.1 NZ_CP092589:738440-738925 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T236712 NZ_LR699570:2246069-2246467 [Mycobacterium tuberculosis variant bovis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT236712 WP_004150912.1 NZ_CP092589:738150-738443 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT236712 NZ_LR699570:2245841-2246068 [Mycobacterium tuberculosis variant bovis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References